patch pipette puller (Sutter Instrument Company)
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Sutter Instrument Company
patch pipette puller
Patch Pipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 2429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/patch-pipette+puller+flaming/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pm38077336-67-18-22
Average 96 stars, based on 2429 article reviews
Patch Pipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 2429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/patch-pipette+puller+flaming/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pm38077336-67-18-22
Average 96 stars, based on 2429 article reviews
patch pipette puller - by Bioz Stars,
2026-10
96/100 stars
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other:Article Title: A 2A adenosine receptor-driven cAMP signaling in olfactory bulb astrocytes is unaffected in experimental autoimmune encephalomyelitis Article Snippet: To stimulate these axons and trigger action potentials, a glass micropipette (GC150TF-10, Harvard Apparatus, USA) pulled with a Article Title: Imaging Light Responses of Targeted Neuron Populations in the Rodent Retina Article Snippet: Electrophysiological recording Extracellular spike recordings were obtained with glass microelectrodes in Article Title: Membrane potential response profiles of CA1 pyramidal cells probed with voltage-sensitive dye optical imaging in rat hippocampal slices reveal the impact of GABA(A)-mediated feed-forward inhibition in signal propagation. Article Snippet: The spatial and temporal distribution of excitatory and inhibitory membrane potential responses on a cell plays an important role in neuronal calculations in local neuronal circuits in the brain.. The electrical dynamics of excitatory and inhibitory inputs along the somatodendritic extent of CA1 pyramidal cells during circuit activation were examined by stimulating strata radiatum (SR), oriens (SO), and lacunosum-moleculare (SLM) and measuring laminar responses with voltage-sensitive dye (VSD) optical recording methods.. We first confirmed the linearity of the optical signal by comparing fluorescence changes in CA1 to global membrane potential changes when slices were bathed in high-potassium ([K]O = 25 mM) solution. Article Title: Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures Article Snippet: Transferring:Article Title: Imaging Light Responses of Targeted Neuron Populations in the Rodent Retina Article Snippet: .. Extracellular spike recordings were obtained with Article Title: Assaying activity-dependent arteriole and capillary responses in brain slices Article Snippet: 9.2.2 Euthanasia • Anesthesia chamber • Large, sharp scissors or guillotine (e.g., Braintree Scientific Nemi Guillotine) • Carcass bag 9.2.3 Dissection • Ice bath for cooling slicing solution • Tissue slicer such as the Compresstome VF-300-0Z Microtome (Precisionary Instruments Inc.) or Vibratome VT1200 S (Leica Biosystems) • Warm water bath (e.g., All Stainless-Steel Water Bath Model 181, Precision Scientific) • Various scissors (e.g., iris scissors, fine scissors) • Forceps (curved forceps with fine tip and blunt forceps) • Spatulas (one flat and one angled end) • Rongeurs bone cutters for adult animals (e.g., #14091, World Precision Instruments) • Slice transfer pipette with large tip opening (DIY: we use plastic 7 mL Pasteur pipettes with the tip cut off to create an opening roughly the size of the brain slices) • Petri dish for dissecting brain (we use Sylgard-coated petri dishes, which makes the bottom of the dish less slippery and improves handling of the tissue) • Cyanoacrylate glue • Small plastic or glass beaker • Razor blade • Slice recovery and storage chambers (see Fig. 2 for DIY instructions) 9.2.4 Imaging • Vibration isolation table (e.g., Newport Optical table) • Faraday cage that fits on the isolation table and surrounds the microscope Neurophotonics 031913-10 Jul–Sep 2022 • Vol. .. 9(3) Downloaded From: https://www.spiedigitallibrary.org/journals/Neurophotonics on 15 Dec 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use • Microscope (e.g., Olympus BX51WI) with DIC optics • Low magnification air objective (e.g., UPlanFL N 4 × ∕0.13, Olympus) and high magnification water-immersion objective (e.g., LUMPlanFL N 40 × ∕0.80 W, Olympus) • Bright-field light source (e.g., Olympus TH4-100) • Epifluorescence light source (e.g., EXFO, X-Cite Series 120) • Camera (e.g., Thor Labs USB 3.0 CMOS Camera or a DAGE-MTI IR-1000 that is more sensitive for IR-DIC imaging) • Patch-clamp amplifier (e.g., Axopatch 200B, Axon Instruments) • Digitizer (e.g., Digidata 1450-B, Axon Instruments) • Isolated current stimulator (e.g., DS3, Digitimer) • Micromanipulators (e.g., MP-225, Sutter Instrument Company) Article Title: Imaging Light Responses of Targeted Neuron Populations in the Rodent Retina Article Snippet: Each retina was fixed after recording (4% paraformaldehyde, 1 h, 20°C), mounted on a glass slide, and counterstained with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories) for confocal imaging [Zeiss 510 laser-scanning microscope; 10×, 0.45 numerical aperture (NA), and 40×, 1.3 NA objectives]. .. Extracellular spike recordings were obtained with Olfactory:Article Title: A 2A adenosine receptor-driven cAMP signaling in olfactory bulb astrocytes is unaffected in experimental autoimmune encephalomyelitis. Article Snippet: .. To stimulate these axons and trigger action potentials, a glass micropipette (GC150TF-10, Harvard Apparatus, USA) pulled with a Slice Preparation:Article Title: A 2A adenosine receptor-driven cAMP signaling in olfactory bulb astrocytes is unaffected in experimental autoimmune encephalomyelitis. Article Snippet: .. To stimulate these axons and trigger action potentials, a glass micropipette (GC150TF-10, Harvard Apparatus, USA) pulled with a Microscopy:Article Title: Assaying activity-dependent arteriole and capillary responses in brain slices Article Snippet: 9.2.2 Euthanasia • Anesthesia chamber • Large, sharp scissors or guillotine (e.g., Braintree Scientific Nemi Guillotine) • Carcass bag 9.2.3 Dissection • Ice bath for cooling slicing solution • Tissue slicer such as the Compresstome VF-300-0Z Microtome (Precisionary Instruments Inc.) or Vibratome VT1200 S (Leica Biosystems) • Warm water bath (e.g., All Stainless-Steel Water Bath Model 181, Precision Scientific) • Various scissors (e.g., iris scissors, fine scissors) • Forceps (curved forceps with fine tip and blunt forceps) • Spatulas (one flat and one angled end) • Rongeurs bone cutters for adult animals (e.g., #14091, World Precision Instruments) • Slice transfer pipette with large tip opening (DIY: we use plastic 7 mL Pasteur pipettes with the tip cut off to create an opening roughly the size of the brain slices) • Petri dish for dissecting brain (we use Sylgard-coated petri dishes, which makes the bottom of the dish less slippery and improves handling of the tissue) • Cyanoacrylate glue • Small plastic or glass beaker • Razor blade • Slice recovery and storage chambers (see Fig. 2 for DIY instructions) 9.2.4 Imaging • Vibration isolation table (e.g., Newport Optical table) • Faraday cage that fits on the isolation table and surrounds the microscope Neurophotonics 031913-10 Jul–Sep 2022 • Vol. .. 9(3) Downloaded From: https://www.spiedigitallibrary.org/journals/Neurophotonics on 15 Dec 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use • Microscope (e.g., Olympus BX51WI) with DIC optics • Low magnification air objective (e.g., UPlanFL N 4 × ∕0.13, Olympus) and high magnification water-immersion objective (e.g., LUMPlanFL N 40 × ∕0.80 W, Olympus) • Bright-field light source (e.g., Olympus TH4-100) • Epifluorescence light source (e.g., EXFO, X-Cite Series 120) • Camera (e.g., Thor Labs USB 3.0 CMOS Camera or a DAGE-MTI IR-1000 that is more sensitive for IR-DIC imaging) • Patch-clamp amplifier (e.g., Axopatch 200B, Axon Instruments) • Digitizer (e.g., Digidata 1450-B, Axon Instruments) • Isolated current stimulator (e.g., DS3, Digitimer) • Micromanipulators (e.g., MP-225, Sutter Instrument Company) Imaging:Article Title: Assaying activity-dependent arteriole and capillary responses in brain slices Article Snippet: 9.2.2 Euthanasia • Anesthesia chamber • Large, sharp scissors or guillotine (e.g., Braintree Scientific Nemi Guillotine) • Carcass bag 9.2.3 Dissection • Ice bath for cooling slicing solution • Tissue slicer such as the Compresstome VF-300-0Z Microtome (Precisionary Instruments Inc.) or Vibratome VT1200 S (Leica Biosystems) • Warm water bath (e.g., All Stainless-Steel Water Bath Model 181, Precision Scientific) • Various scissors (e.g., iris scissors, fine scissors) • Forceps (curved forceps with fine tip and blunt forceps) • Spatulas (one flat and one angled end) • Rongeurs bone cutters for adult animals (e.g., #14091, World Precision Instruments) • Slice transfer pipette with large tip opening (DIY: we use plastic 7 mL Pasteur pipettes with the tip cut off to create an opening roughly the size of the brain slices) • Petri dish for dissecting brain (we use Sylgard-coated petri dishes, which makes the bottom of the dish less slippery and improves handling of the tissue) • Cyanoacrylate glue • Small plastic or glass beaker • Razor blade • Slice recovery and storage chambers (see Fig. 2 for DIY instructions) 9.2.4 Imaging • Vibration isolation table (e.g., Newport Optical table) • Faraday cage that fits on the isolation table and surrounds the microscope Neurophotonics 031913-10 Jul–Sep 2022 • Vol. .. 9(3) Downloaded From: https://www.spiedigitallibrary.org/journals/Neurophotonics on 15 Dec 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use • Microscope (e.g., Olympus BX51WI) with DIC optics • Low magnification air objective (e.g., UPlanFL N 4 × ∕0.13, Olympus) and high magnification water-immersion objective (e.g., LUMPlanFL N 40 × ∕0.80 W, Olympus) • Bright-field light source (e.g., Olympus TH4-100) • Epifluorescence light source (e.g., EXFO, X-Cite Series 120) • Camera (e.g., Thor Labs USB 3.0 CMOS Camera or a DAGE-MTI IR-1000 that is more sensitive for IR-DIC imaging) • Patch-clamp amplifier (e.g., Axopatch 200B, Axon Instruments) • Digitizer (e.g., Digidata 1450-B, Axon Instruments) • Isolated current stimulator (e.g., DS3, Digitimer) • Micromanipulators (e.g., MP-225, Sutter Instrument Company) Patch Clamp:Article Title: Assaying activity-dependent arteriole and capillary responses in brain slices Article Snippet: 9.2.2 Euthanasia • Anesthesia chamber • Large, sharp scissors or guillotine (e.g., Braintree Scientific Nemi Guillotine) • Carcass bag 9.2.3 Dissection • Ice bath for cooling slicing solution • Tissue slicer such as the Compresstome VF-300-0Z Microtome (Precisionary Instruments Inc.) or Vibratome VT1200 S (Leica Biosystems) • Warm water bath (e.g., All Stainless-Steel Water Bath Model 181, Precision Scientific) • Various scissors (e.g., iris scissors, fine scissors) • Forceps (curved forceps with fine tip and blunt forceps) • Spatulas (one flat and one angled end) • Rongeurs bone cutters for adult animals (e.g., #14091, World Precision Instruments) • Slice transfer pipette with large tip opening (DIY: we use plastic 7 mL Pasteur pipettes with the tip cut off to create an opening roughly the size of the brain slices) • Petri dish for dissecting brain (we use Sylgard-coated petri dishes, which makes the bottom of the dish less slippery and improves handling of the tissue) • Cyanoacrylate glue • Small plastic or glass beaker • Razor blade • Slice recovery and storage chambers (see Fig. 2 for DIY instructions) 9.2.4 Imaging • Vibration isolation table (e.g., Newport Optical table) • Faraday cage that fits on the isolation table and surrounds the microscope Neurophotonics 031913-10 Jul–Sep 2022 • Vol. .. 9(3) Downloaded From: https://www.spiedigitallibrary.org/journals/Neurophotonics on 15 Dec 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use • Microscope (e.g., Olympus BX51WI) with DIC optics • Low magnification air objective (e.g., UPlanFL N 4 × ∕0.13, Olympus) and high magnification water-immersion objective (e.g., LUMPlanFL N 40 × ∕0.80 W, Olympus) • Bright-field light source (e.g., Olympus TH4-100) • Epifluorescence light source (e.g., EXFO, X-Cite Series 120) • Camera (e.g., Thor Labs USB 3.0 CMOS Camera or a DAGE-MTI IR-1000 that is more sensitive for IR-DIC imaging) • Patch-clamp amplifier (e.g., Axopatch 200B, Axon Instruments) • Digitizer (e.g., Digidata 1450-B, Axon Instruments) • Isolated current stimulator (e.g., DS3, Digitimer) • Micromanipulators (e.g., MP-225, Sutter Instrument Company) Isolation:Article Title: Assaying activity-dependent arteriole and capillary responses in brain slices Article Snippet: 9.2.2 Euthanasia • Anesthesia chamber • Large, sharp scissors or guillotine (e.g., Braintree Scientific Nemi Guillotine) • Carcass bag 9.2.3 Dissection • Ice bath for cooling slicing solution • Tissue slicer such as the Compresstome VF-300-0Z Microtome (Precisionary Instruments Inc.) or Vibratome VT1200 S (Leica Biosystems) • Warm water bath (e.g., All Stainless-Steel Water Bath Model 181, Precision Scientific) • Various scissors (e.g., iris scissors, fine scissors) • Forceps (curved forceps with fine tip and blunt forceps) • Spatulas (one flat and one angled end) • Rongeurs bone cutters for adult animals (e.g., #14091, World Precision Instruments) • Slice transfer pipette with large tip opening (DIY: we use plastic 7 mL Pasteur pipettes with the tip cut off to create an opening roughly the size of the brain slices) • Petri dish for dissecting brain (we use Sylgard-coated petri dishes, which makes the bottom of the dish less slippery and improves handling of the tissue) • Cyanoacrylate glue • Small plastic or glass beaker • Razor blade • Slice recovery and storage chambers (see Fig. 2 for DIY instructions) 9.2.4 Imaging • Vibration isolation table (e.g., Newport Optical table) • Faraday cage that fits on the isolation table and surrounds the microscope Neurophotonics 031913-10 Jul–Sep 2022 • Vol. .. 9(3) Downloaded From: https://www.spiedigitallibrary.org/journals/Neurophotonics on 15 Dec 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use • Microscope (e.g., Olympus BX51WI) with DIC optics • Low magnification air objective (e.g., UPlanFL N 4 × ∕0.13, Olympus) and high magnification water-immersion objective (e.g., LUMPlanFL N 40 × ∕0.80 W, Olympus) • Bright-field light source (e.g., Olympus TH4-100) • Epifluorescence light source (e.g., EXFO, X-Cite Series 120) • Camera (e.g., Thor Labs USB 3.0 CMOS Camera or a DAGE-MTI IR-1000 that is more sensitive for IR-DIC imaging) • Patch-clamp amplifier (e.g., Axopatch 200B, Axon Instruments) • Digitizer (e.g., Digidata 1450-B, Axon Instruments) • Isolated current stimulator (e.g., DS3, Digitimer) • Micromanipulators (e.g., MP-225, Sutter Instrument Company) Introduce:Article Title: Assaying activity-dependent arteriole and capillary responses in brain slices Article Snippet: 9.2.2 Euthanasia • Anesthesia chamber • Large, sharp scissors or guillotine (e.g., Braintree Scientific Nemi Guillotine) • Carcass bag 9.2.3 Dissection • Ice bath for cooling slicing solution • Tissue slicer such as the Compresstome VF-300-0Z Microtome (Precisionary Instruments Inc.) or Vibratome VT1200 S (Leica Biosystems) • Warm water bath (e.g., All Stainless-Steel Water Bath Model 181, Precision Scientific) • Various scissors (e.g., iris scissors, fine scissors) • Forceps (curved forceps with fine tip and blunt forceps) • Spatulas (one flat and one angled end) • Rongeurs bone cutters for adult animals (e.g., #14091, World Precision Instruments) • Slice transfer pipette with large tip opening (DIY: we use plastic 7 mL Pasteur pipettes with the tip cut off to create an opening roughly the size of the brain slices) • Petri dish for dissecting brain (we use Sylgard-coated petri dishes, which makes the bottom of the dish less slippery and improves handling of the tissue) • Cyanoacrylate glue • Small plastic or glass beaker • Razor blade • Slice recovery and storage chambers (see Fig. 2 for DIY instructions) 9.2.4 Imaging • Vibration isolation table (e.g., Newport Optical table) • Faraday cage that fits on the isolation table and surrounds the microscope Neurophotonics 031913-10 Jul–Sep 2022 • Vol. .. 9(3) Downloaded From: https://www.spiedigitallibrary.org/journals/Neurophotonics on 15 Dec 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use • Microscope (e.g., Olympus BX51WI) with DIC optics • Low magnification air objective (e.g., UPlanFL N 4 × ∕0.13, Olympus) and high magnification water-immersion objective (e.g., LUMPlanFL N 40 × ∕0.80 W, Olympus) • Bright-field light source (e.g., Olympus TH4-100) • Epifluorescence light source (e.g., EXFO, X-Cite Series 120) • Camera (e.g., Thor Labs USB 3.0 CMOS Camera or a DAGE-MTI IR-1000 that is more sensitive for IR-DIC imaging) • Patch-clamp amplifier (e.g., Axopatch 200B, Axon Instruments) • Digitizer (e.g., Digidata 1450-B, Axon Instruments) • Isolated current stimulator (e.g., DS3, Digitimer) • Micromanipulators (e.g., MP-225, Sutter Instrument Company) |